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Concentration and Quality Control

This service is for customers who intend to run sequencing in the CTG facility

RNA concentration and purity

NanoDrop is used to measure concentration and purity of nucleic acids before starting a sequencing project. RNA dissolved in water should have OD260/280 close to 2.0 and OD260/230 preferably above 2. Lower ratios might indicate on contamination. A low OD260/230 reading can also occur as an artefact in samples with low concentration (< 50 ng/µl)

RNA quality

Caliper LabChip GX and Agilent 2100 Bioanalyzer are used to analyze the integrity of nucleic acids. The RNA Quality Score (RQS) for Caliper and RNA Integrity Number (RIN) for Bioanalyzer are software algorithms allowing the classification of total RNA, based on a numbering system from 1 to 10, with 1 being the most degraded and 10 being the most intact. Total RNA for expression studies needs to be of high and even quality.

DNA (FFPE) quality

 

 

 

 

 

 

 

 

 

 

 

 

Page Manager:

Contact for service

E-mail: CTGservice [at] med [dot] lu [dot] se
Ingrid Wilson
  Phone: +46706855137
Markus Heidenblad
  Phone: +4646173592

Nanodrop
nanodrop

Agilent 2100 Bioanalyzer
Agilent 2100 Bioanalyzer

Caliper LabChip GX
Caliper LabChip GX

Contact CTG:  

   Phone: +46706855137 
   E-mail: CTGservice [at] med [dot] lu [dot] se

Visiting Addresses:

   Medicon Village 404:B3                                BMC - Biomedical Centre
   Scheelevägen 2                                             Sölvegatan 23 B
         SE-223 81 Lund                                             SE - 221 85 Lund

         Link to map for Medicon Village                  Link to map for BMC