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DNA Library Preparation and Sequencing

Our DNA service includes library preparation for whole genome (WGS) and exome (WES) sequencing, or a subset of genes or regions, and sequencing on Illumina sequencing platforms. Contact CTG for a project meeting, where we can discuss which strategy will suit your project.

Plasmids and Small Genomes

Plasmid and small genome library preparation is performed using Illumina’s Nextera XT Kit. Consult Illumina’s web page for detailed information about this kit.

Whole Genome Sequencing

Whole genome library preparation is performed using Illumina’s TruSeq DNA PCR-Free Library Prep Kit. Consult Illumina’s web page for detailed information about this kit.

Exome Sequencing

Exome library preparation is performed using Roche's KAPA HyperPrep Kit (LÄNK: https://sequencing.roche.com/en/products-solutions/by-category/library-preparation/dna-library-preparation/kapa-hyperprep.html) and exome capture probes from TWIST  Bioscience (LÄNK: https://www.twistbioscience.com/products/ngs#product-featured-1951).

Targeted Gene Sequencing

Ready-made or customized gene panels, either capture- or amplicon-based can be ordered. Contact CTG for a project meeting, where we can discuss which kits that will suit your particular project.

As part of the Genomic Medicine Sweden (GMS) initiative, CTG is involved in the development of gene panels for e.g. haematology and solid tumours. Currently, these capture-based panels are acquired from TWIST Bioscience https://www.twistbioscience.com/products/ngs and can also be used for suitable research projects. Ordering a customized panel from TWIST is also an option.

Page Manager:

Contact for service

E-mail: CTGservice [at] med [dot] lu [dot] se
Ingrid Wilson
  Phone: +46706855137
Markus Heidenblad
  Phone: +4646173592

Covaris
Covaris

Whole exome sequencing variant interpretation and visualization tools
Whole exome sequencing variant interpretation and visualization tools

Contact CTG:  

   Phone: +46706855137 
   E-mail: CTGservice [at] med [dot] lu [dot] se

Visiting Addresses:

   Medicon Village 404:B3                                BMC - Biomedical Centre
   Scheelevägen 2                                             Sölvegatan 23 B
         SE-223 81 Lund                                             SE - 221 85 Lund

         Link to map for Medicon Village                  Link to map for BMC