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DNA Library Preparation and Sequencing

Whole Genome Sequencing


Our DNA service includes whole genome library preparation and sequencing on Illumina sequencing platforms.
Library preparation is performed using:

Whole Exome Sequencing and Targeted Gene Sequencing

Our DNA service includes library preparation for exome sequencing (WES) and targeted gene sequencing. Libraries will be sequenced on Illumina sequencing platforms.

Library preparation is performed using:

Hybridization is performed using:

Whole Exome Sequencing

Targeted Gene Sequencing

Of-the-shelf or customized gene panels, either capture- or amplicon-based can be ordered. You can customize your own hybridization panel for your project. Contact CTG service for a project meeting.

  • hybrid capture using custom or of the shelf capture probes from TWIST Bioscience.

Small Genome Sequencing

Our DNA service includes small genome library preparation for microbiology applications and sequencing on Illumina sequencing platsforms.

Library preparation is performed using:

General information before starting a DNA Sequencing project

  • The DNA should be of high quality and diluted in water or EB buffer (presence of EDTA in the buffer might affect the library preparation).
  • DNA isolated from FFPE samples must be of good quality, QC needs to be performed by a qPCR-based method (available at CTG).
  • DNA should ideally have an OD260/280 of 1.8-2and OD260/230 of 1.8-2.2.
  • All samples should be diluted/normalized based on concentration measurements by a fluorometric method (e.g. Qubit). Measurements based on spectrophotometric method (e.g. Nanodrop) are not acceptable.
  • You need to specify the sequencing depth for each sample.

For WES and Targeted Gene Panels:

  • Up to eight samples are pooled prior to the hybrid-capture.
  • The price is calculated per pool. (For example, if you have 9 samples this would be calculated as two pools).
  • If you are interested in custom probe pools for targeted sequencing, contact us for more information.

Sample Requirement

  • DNA input amounts differ between assays. Please see table below for required sample concentration and delivery volumes.
  • Always dilute samples in nuclease-free H2O or EB buffer.

Lower concentrations and volumes can be discussed.

Delivery of samples to CTG

DNA should be delivered aliquoted on ice in:

  • a strip labeled with Sample ID or Sample no. (ie. 1, 2, 3...) on lid and side of tube

or

  • a fully skirted 0,2 ml plate with cold resistant seal - can be provided by CTG (place samples column wise, A1, A2…)

Applications under development

  • Whole genome sequencing workflow suitable for DNA isolated from FFPET samples.
  • Targeted gene sequencing workflow for ctDNA analysis.

Contact

Development of protocols

If you are interested in collaborating with us on our protocols under development, or if you have a suggestion for the implementation or development of a novel method, please contact tina [dot] catela_ivkovic [at] med [dot] lu [dot] se (Tina Catela Ivkovic).

Available services

To initiate a project using our available services, please start a project in iLab. ILab is a managing software for core facilities and increases the transparency of projects and quotes.
We have several user guides available. A quick guide for PIs to set up a group/lab, a starting guide for further lab members and a more detailed guide providing information on all functions in iLab. If you have any questions regarding iLab please write to CTGiLAB [at] med [dot] lu [dot] se.

Using iLab

You can login using your LucatID on the main Lund University iLab webpage.
You can also use this link, which brings you directly to our Service Request site after login.

Consultation and preliminary quotes

If you want to schedule a meeting to discuss your project or want to get a preliminary quote, choose “Consultation”, fill out the form, save it and click on “send request” at the bottom of the page. We will process your request as soon as possible.


If you are ready to start a project click on one of the available project request options, fill out the Contact & Invoicing Information as well as project specific forms, save them and click “send request” at the bottom of the page. We will check the forms and send you a quote.

 

Contact for service

CTGservice [at] med [dot] lu [dot] setarget="_self"

Whole exome sequencing variant interpretation and visualization tools
Whole exome sequencing variant interpretation and visualization tools