DNA Library Preparation and Sequencing
Plasmids and Small Genomes
Plasmid and small genome library preparation is performed using Illumina’s Nextera XT Kit. Consult Illumina’s web page for detailed information about this kit.
Whole Genome Sequencing
Whole genome library preparation is performed using Illumina’s TruSeq DNA PCR-Free Library Prep Kit. Consult Illumina’s web page for detailed information about this kit.
Exome library preparation is performed using Roche's KAPA HyperPrep Kit (LÄNK: https://sequencing.roche.com/en/products-solutions/by-category/library-preparation/dna-library-preparation/kapa-hyperprep.html) and exome capture probes from TWIST Bioscience (LÄNK: https://www.twistbioscience.com/products/ngs#product-featured-1951).
Targeted Gene Sequencing
Ready-made or customized gene panels, either capture- or amplicon-based can be ordered. Contact CTG for a project meeting, where we can discuss which kits that will suit your particular project.
As part of the Genomic Medicine Sweden (GMS) initiative, CTG is involved in the development of gene panels for e.g. haematology and solid tumours. Currently, these capture-based panels are acquired from TWIST Bioscience https://www.twistbioscience.com/products/ngs and can also be used for suitable research projects. Ordering a customized panel from TWIST is also an option.