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Sample Requirement

DNA amount recommended 

Fluorometric-based method (Qubit or equivalent)

Lower concentrations and volumes can be discussed.

Analysis/kit

Diluted inAmountVolume

 

Plasmids and Small Genomes

Nextera XT

 

 

EB  buffer

 

200 ng

 

20 μL

Whole Genome Sequencing

TruSeq Nano DNA

Insert size 350 bp

 

Nuclease-free ultrapure H2O, low TE buffer (0,1 mM EDTA)or EB buffer

 

300 ng

 

30 μL

Whole Genome Sequencing

TruSeq DNA PCR-Free

Insert size 350 bp


Nuclease-free ultrapure H2O, low TE buffer (0,1 mM EDTA) or EB buffer


3000 ng


60 μL


Whole Exome Sequencing

 


Nuclease-free ultrapure H2O, low TE buffer (0,1 mM EDTA) or EB buffer


500 ng


50 μL


Gene panel, gDNA


Nuclease-free ultrapure H2O or EB buffer

 


500 ng

50 μL


Gene panel, FFPE-DNA*

 


EB buffer

 


200 ng


20 μL


* An initial QC step by qPCR is required for FFPE-DNA to determine if the DNA quality is sufficient for library preparation.

 

Page Manager:

Contact for service

E-mail: CTGservice [at] med [dot] lu [dot] se
Ingrid Wilson
  Phone: +46706855137
Markus Heidenblad
  Phone: +4646173592

CTG - Center for Translational Genomics Excellent Infrastructure at Lund University - Roll-up with Lund University logotype and seal over a blue DNA-string

Contact CTG:  

   Phone: +46706855137 
   E-mail: CTGservice [at] med [dot] lu [dot] se

Visiting Addresses:

   Medicon Village 404:B3                                BMC - Biomedical Centre
   Scheelevägen 2                                             Sölvegatan 23 B
         SE-223 81 Lund                                             SE - 221 85 Lund

         Link to map for Medicon Village                  Link to map for BMC