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RNA Library Preparation and Sequencing

Our RNA service includes library preparation and sequencing. Inquire for other kits than below.

Concentration check

Delivered RNA concentration is verified using a fluorometric assay.

Library preparation

Library concentration and quality check

Concentration of cDNA libraries is measured using a fluorometric assay.

Quality and size calculation of libraries is performed on a micro-capillary gel electrophoresis system.

Sequencing

llumina’s NextSeq 500 is used for sequencing of libraries in addition with the NextSeq®500/550 High Output Kit v2 (kits with different number of cycles and output are available). A sequencing metrics check is done after the run.

Data delivery

See Bioinformatics and Deliverables.

Page Manager:

Contact for service

E-mail: CTGservice [at] med [dot] lu [dot] se
Ingrid Wilson
  Phone: +46706855137
Markus Heidenblad
  Phone: +4646173592

Lab personnel in front of machine King Fisher Flex
King Fisher Flex

NextSeq 500
NextSeq 500

Seq Complete

Contact CTG:  

   Phone: +46706855137 
   E-mail: CTGservice [at] med [dot] lu [dot] se

Visiting Addresses:

   Medicon Village 404:B3                                BMC - Biomedical Centre
   Scheelevägen 2                                             Sölvegatan 23 B
         SE-223 81 Lund                                             SE - 221 85 Lund

         Link to map for Medicon Village                  Link to map for BMC