RNA Library Preparation and Sequencing
Delivered RNA concentration is verified using a fluorometric assay.
- Illumina TruSeq®Stranded mRNA - polyA enrichment and strand-specific cDNA synthesis.
- Illumina TruSeq®Stranded total RNA - rRNA depletion and strand-specific cDNA synthesis.
- SMARTer Stranded Total RNA-Seq v2 Pico Input Mammalian - rcDNA depletion and strand-specific cDNA synthesis of FFPE or Total RNA
Library concentration and quality check
Concentration of cDNA libraries is measured using a fluorometric assay.
Quality and size calculation of libraries is performed on a micro-capillary gel electrophoresis system.
llumina’s NextSeq 500 is used for sequencing of libraries in addition with the NextSeq®500/550 High Output Kit v2 (kits with different number of cycles and output are available). A sequencing metrics check is done after the run.