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Sample Requirement

Concentration and Purity

Use spectrophotometrical method to get the concentration and a ratio (OD260/280 ~2.0 and OD260/230 ~1.8-2.2) of each sample (the ratio shows purity of samples).

Before delivery measure diluted RNA fluorometricly.

 Quality

Use Agilent Technologies 2100 Bioanalyzer (RIN) or equivalent equipment  for RNA quality/integrity check.

  • mRNA analysis: RIN ≥ 8. If any sample has lower quality score please consult us.
  • total RNA analysis: RIN value is not as crucial. RIN>3.
  • FFPE RNA analysis: DV200 value < 30% is NOT recommended as RNA is too degraded. 

Freeze and thaw samples as few times as possible and work with the RNA on ice to avoid further degradation.

Total RNA amount recommended 

Always dilute samples in nuclease-free ultrapure H2O.

Analysis/kit

Recommended

Conc.

Vol.

Illumina mRNA analysis

10 ng/μL

110 μL

Illumina TotalRNA analysis

50 ng/μL

25 μL

SMARTer TotalRNA Pico analysis

Should be discussed and
adjusted according to
RNA amount available.

20 uL

Lower concentrations and volumes can be discussed.

Delivery to CTG

  • Order Form and Sample sheet (provided by CTG) have to be filled in and sent to facility by email (CTGservice [at] med [dot] lu [dot] se) together with  “QC PDF” (PDF of electropherogram).
  • Use Checklist for RNA delivery (provided by CTG).
Page Manager:

Contact for service

E-mail: CTGservice [at] med [dot] lu [dot] se
Ingrid Wilson
  Phone: +46706855137
Markus Heidenblad
  Phone: +4646173592

Tubes for delivery of samples
Tubes for delivery of samples

Contact CTG:  

   Phone: +46706855137 
   E-mail: CTGservice [at] med [dot] lu [dot] se

Visiting Addresses:

   Medicon Village 404:B3                                BMC - Biomedical Centre
   Scheelevägen 2                                             Sölvegatan 23 B
         SE-223 81 Lund                                             SE - 221 85 Lund

         Link to map for Medicon Village                  Link to map for BMC