Library concentration and quality check
We recommend you to do the following steps before pooling your libraries:
- Verify concentration of libraries using a fluorometric assay.
- Perform quality and size calculation of libraries on a micro-capillary gel electrophoresis system.
- Verify concentration of pool using a fluorometric assay before delivery.
- Calculate molarity of pool using pool concentration and average library size.
For details see Bioinformatics and Deliverables