Molarity and amounts of library pool for sequencing

Dilute library pool in EB buffer 10mM Tris-Cl, pH 8,5. Lower concentrations than stated below can be discussed.

For NextSeq:

• Molarity of pool: 2 nM
• Volume: 50 ul

For NovaSeq:

• Molarity and volume depend on type of library and flowcell
• Unique dual index

For MiSeq and MiniSeq:

• Molarity of pool: 4 nM
• Volume: 50 ul

Delivery to CTG

• Fill in OrderForm, pool information, and SampleSheet for Illumina (csv file) and send to your contact at CTG.