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Sample Requirement

Molarity and amounts of library pool for sequencing

Dilute library pool in EB buffer 10mM Tris-Cl, pH 8,5. Lower concentrations than stated below can be discussed.

For NextSeq:

  • Molarity of pool: 2 nM
  • Volume: 50 ul

For NovaSeq:

  • Molarity and volume depend on type of library and flowcell
  • Unique dual index

For MiSeq and MiniSeq:

  • Molarity of pool: 4 nM
  • Volume: 50 ul

Delivery to CTG

  • Fill in OrderForm, pool information, and SampleSheet for Illumina (csv file) and send to your contact at CTG.

 

Page Manager:

Contact for service

E-mail: CTGservice [at] med [dot] lu [dot] se
Ingrid Wilson
  Phone: +46706855137
Markus Heidenblad
  Phone: +4646173592

CTG - Center for Translational Genomics Excellent Infrastructure at Lund University - Roll-up with Lund University logotype and seal over a blue DNA-string

Contact CTG:  

   Phone: +46706855137 
   E-mail: CTGservice [at] med [dot] lu [dot] se

Visiting Addresses:

   Medicon Village 404:B3                                BMC - Biomedical Centre
   Scheelevägen 2                                             Sölvegatan 23 B
         SE-223 81 Lund                                             SE - 221 85 Lund

         Link to map for Medicon Village                  Link to map for BMC