Single-Cell and Spatial Genomics Platform
Established in 2019, CTG offers a dynamic, state-of-the-art platform for single-cell sequencing to users. The aim is to provide expertise, accessibility, proximity and service to researchers in need of single-cell genomics.
General guidelines
Consultations and Service Projects, as well as a Booking Schedule are available in iLab (see “How to start a project”)
Gentle sample isolation is essential to prevent bias against vulnerable cell types while fast isolation is desirable to reduce degradation of nucleic acids.
Thus, prior to initiating a project at the Single-Cell Genomics Platform, users are asked to thoroughly evaluate a cell or nucleus isolation strategy suitable for their target tissue and research question. Users are also strongly encouraged to evaluate their sample quality (e.g. RNA integrity) prior to submitting single-cell genomics work at the facility.
Once a project is accepted by the Single-Cell Genomics Platform, users must follow sample preparation and delivery instructions provided by the platform.
Available services
scTranscriptomics
- Single-cell Gene Expression (3´GEX) library preparation (10x Genomics, v3.1, scmRNAseq)
- CITE-Seq
- Hashing
- CRISPR Screen
- Single-cell Immune Profiling library preparation (10x Genomics, v2, scVDJ)
- Full-length TCR/BCR sequences
- Feature Barcode
- Single-cell Multiome ATAC + Gene Expression (10x Genomics, scMultiome)
scTranscriptomics (fixed cells/nuclei)
- Single-cell Gene Expression Flex Profiling (10x Genomics, v.1, Flex, custom probe design)
- Cell Surface Protein
- Evercode™ WT (Parse Bioscience, Parse)
scEpigenomics
- Single-cell ATAC-seq library preparation (10x Genomics, v2, scATAC)
- Single-cell Multiome ATAC + Gene Expression (10x Genomics, scMultiome)
Cell isolation
- High precision cell isolation of sparse biological samples using CellenOne F1.4.
Spatial transcriptomics
- Spatial transcriptomics on FFPE fixed specimen using Visium CytAssist
Equipment
- 10x Genomics Chromium Controller
- 10x Genomics Chromium X
- 10x Genomics Visium CytAssist
- Scienion CellenOne F1.4
- Nexcelom Cellaca Mx
- Illumina Sequencer NovaSeq 6000, NextSeq 2000, NovaSeq X Plus
Applications under development
- Single-cell proteomics using the proteochip on the CellenOne F1.4.
- Customization of existing 10x Genomics applications (e.g. DogmaSeq, viral barcode capturing, integration with long-read technology using Oxford Nanopore).
If you are interested in collaborating with us for our protocols under development, or if you have a suggestion for the implementation or development of a novel method, please contact Julia Bräunig (singlecell [dot] ctgservice [at] med [dot] lu [dot] se (singlecell[dot]ctgservice[at]med[dot]lu[dot]se)).
Requests for service/consults:
Link to iLab
Contact Information
ctg_service [at] med [dot] lu [dot] se
singlecell [dot] ctgservice [at] med [dot] lu [dot] se
Phone
+46 73 075 3563
+46 73 096 8048
Address
BMC - Biomedical Centre D14
Klinikgatan 32
221 85 Lund