RNA concentration

RNA Concentration is measured by a fluorometric method, using the Qubit 4 or Qubit Flex instruments.

Available Assays:

• Broad Range RNA Assay
• High Sensitivity RNA Assay

RNA quality

RNA quality is assessed by micro-capillary gel electrophoresis, using the TapeStation 4200. RNA sample quality is expressed as RNA Integrity Number (RIN) that can vary from 1 to 10, with the higher number indicating higher quality. 

Available assays:

• RNA Assay
• High Sensitivity RNA Assay

Sample Requirements

Detailed instructions will be provided after requesting a QC project in iLab. 

Deliverables

Concentration measurement results will be delivered as:

• Excel file containing the measured values.

Quality measurement results will be delivered as:

• PDF files containing electropherogram and RIN values.
• Excel file containing RIN values.

DNA concentration

DNA Concentration is measured by a fluorometric method, using the Qubit 4 or Qubit Flex instruments.

Available Assays:

• Broad Range dsDNA Assay
• High Sensitivity dsDNA Assay

DNA Quality

DNA quality is assessed by micro-capillary gel electrophoresis, using the TapeStation 4200. DNA sample quality is expressed as DNA Integrity Number (RIN) that can vary from 1 to 10, with the higher number indicating higher quality. 

Available assays:

• Genomic DNA Assay
• Cell-free DNA Assay

Sample Requirements

Detailed instructions will be provided after requesting a QC project in iLab. 

Deliverables

Concentration measurement results will be delivered as:

• Excel file containing the measured values.

Quality measurement results will be delivered as:

• PDF files containing electropherogram and DIN values.
• Excel file containing DIN values.

DNA Quality for FFPE samples

In addition to concentration measurements described for DNA above, a qPCR is performed to assess the quality of FFPET-derived DNA more accurately. This assay gives an estimation of DNA fragmentation status and its amplification potential expressed as ∆Cq value.

Sample requirements

• Sample concentration needs to be determined by a fluorometric method (e.g. Qubit).
• Sample needs to be normalized in EB-buffer (10 mM Tris-Cl, pH 8.5).
  • Concentration: 2.5 ng/µl*
  • Volume: 5 µl
    * Samples with concentrations lower than 2.5 ng/µl cannot be processed
• Samples should be delivered in 200 µl strips.
• All samples should be labeled individually, directly on the side and the lid of a tube.
• Samples must be delivered on ice.

Deliverables

Quality measurement results for FFPE samples will be delivered as:

• Excel file containing ∆Cq scores and the quality estimates.